250 Non-invasive diagnosis of early and advanced vulvar lichen sclerosus by multimodal optical coherence tomography

Vulvar Lichen Sclerosus (VLS) is a chronically inflammatory disorder that requires early diagnosis and long-term follow-up for successful therapy prevention of malignancy. In this paper, we propose multimodal optical coherence tomography (MM OCT) as non-invasive diagnostic technique advanced VLS. The MM OCT provides 3D real-time visualization the vulvar skin structure with high spatial resolution, well label-free blood lymphatic vessels. Normal according to characterized by layered structure, clear boundary between epidermis dermis. Dermis contains inclusions background signal corresponding A dense lymph vessels networks are visualized angiography. Early VLS moderate thickness intensity due backscattering stratum corneum. has structures such thickened collagen fibers, dilated cells. dermal–epidermal junction less contrasting. Decreased density detected Primarily, subepidermal disappear. Advanced atrophic scattering low contrast; disrupted. dermis loses its usual formation pathognomonic areas homogenization does not backscatter light looks like signal. Blood on ability structural changes in stages been demonstrated first time.